Journal: Cell Reports Medicine
Article Title: Local delivery of IL-15 and anti-PD-L1 nanobody by in vitro- transcribed circILNb elicits superior antitumor immunity in cold tumors
doi: 10.1016/j.xcrm.2025.102413
Figure Lengend Snippet: circILNb reprograms tumor-specific tdLN and tumor CD8 + T cells (A) (Left) Timeline of the experiment designed to evaluate the p-STAT5 levels of CD8 + T cells in tdLN and (right) legends and abbreviations for different treatment groups. B16F10-OVA tumor-bearing mice were intratumorally injected with circIL, circNb, and circILNb (0.5 mg/kg circRNA per mouse). The circLuc was used as control circScram. p value was determined by two-tailed unpaired t test. Data are mean ± SEM. (B) (Left) Representative sample histogram of pSTAT5 after circRNAs treatment. Summary data for (middle) pSTAT5 MFI and (right) pSTAT5 + cells. Gated on live CD3 + CD8 + cells ( n = 3). (C) Timeline of the experiment designed to evaluate the population and activity of CD8 + T cells in tdLN or tumor. B16F10-OVA tumor-bearing mice were intratumorally injected with circIL, circNb, and circILNb (0.5 mg/kg circRNA per mouse). circLuc was used as control circScram. p value was determined by two-tailed unpaired t test. Data are mean ± SEM. (D) (Left) Representative sample histogram of ki67 expression and (right) quantification of the ki67 MFI of tdLN CD8 + T cells. Gated on live CD3 + CD8 + cells ( n = 5). (E) Counts of tetramer + cells in the tdLN, gated on live CD8 + CD44 + tetramer + cells ( n = 5). (F) The tdLN frequency of Ttsm cells, gated on live CD8 + CD44 + tetramer + TCF1 + TOX − cells ( n = 3). (G) (Left) Representative contour plots, (middle) frequency, and (right) counts of Tsle in tumor, gated on live CD8 + tetramer + PD1 + TCF1 + TIM3 - cells ( n = 4). (H) (Left) Frequency and (right) counts of tetramer + cells in the tumor, gated on live CD8 + cells ( n = 3). (I) Counts of IFN-γ + cells in the tumor, gated on live CD8 + tetramer + cells ( n = 5). (J) Counts of GrzmB + cells in the tumor, gated on live CD8 + tetramer + cells ( n = 5). " n " indicates biologically independent samples.
Article Snippet: PE/Cy7 anti-mouse TCF1 (clone: C63D9) , Cell Signaling Technology , Cat#90511S; RRID: AB_3086656.
Techniques: Injection, Control, Two Tailed Test, Activity Assay, Expressing
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